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目的 采用氟与微量元素联合配制成以钙、磷为基础 ,含有氟和微量元素的矿化液 ,探讨该矿化液对致龋毒力因子——变形链球菌和远缘链球菌的胞外葡糖基转移酶 (GTF)和乳酸脱氢酶 (L DH )的影响。方法 选用国际标准菌株 ATCC S.mutans 2 5 175和 S.Sobrinus6 715为实验菌侏 ,采用厌氧菌的连续培养技术培养细菌 ,用微量酶化学分析方法检测矿化液对 GTF和 L DH酶活性的影响 ,用菌液的吸光度(ABS)来描述。结果 无氟矿化液对 GTF酶活性无明显的抑制作用 (P>0 .0 5 ) ,而含氟矿化液和氟微量元素矿化液对 GTF酶活性有明显抑制作用 (P<0 .0 1) ,两两比较 ,尤以氟微量元素矿化液的作用最强 ;氟微量元素矿化液对 L DH酶活性具有明显的抑制作用 (P<0 .0 1) ,而无氟矿化液和含氟矿化液对其无明显的统计学意义 (P>0 .0 5 )。结论 氟微量元素矿化液比单氟矿化液具有更好的抑制致龋毒力因子 GTF和L DH酶活性的作用。提示氟与微量元素的联合应用 ,是其有效的防龋途径之一 ,同时在调节菌斑生态平衡中起重要作用。
Objective To prepare the mineralized solution containing fluorine and trace elements based on calcium and phosphorus based on the combination of fluorine and trace elements and explore the effect of the mineralized solution on the extracellular load of cariogenic virulence factors Streptococcus mutans and Streptococcus sobrinus Glucosyltransferase (GTF) and lactate dehydrogenase (L DH). Methods The international standard strains ATCC S. mutans 2 5 175 and S.Sobrinus 6 715 were selected as experimental strains for the culture of bacteria with continuous culture of anaerobic bacteria. Trace enzyme chemical analysis was used to detect the activity of mineralization solution on GTF and L DH enzymes The effect is described by the absorbance of the bacterium (ABS). Results Fluoride-free mineralized fluid had no significant inhibitory effect on the activity of GTF (P> 0.05), while the fluorinated mineralized fluid and the mineralized solution of Fluorine and trace element mineral oil significantly inhibited the activity of GTF (P <0. 0 1), any pair of comparisons, especially fluoride trace element mineralization liquid strongest; fluorine trace element mineralized liquid L DH enzyme activity was significantly inhibited (P <0. There was no significant difference between the fluid and fluoride mineralized fluid (P> 0.05). CONCLUSION Fluorine and trace element mineralized solution has a better effect on inhibiting the activity of cariogenic virulence factors GTF and L DH than monofluorine mineralized solution. Prompt joint use of fluorine and trace elements, is one of its effective anti-caries pathways, and plays an important role in the regulation of plaque ecological balance.