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目的 建立快速特异的HBV基因变异检测技术及变异株的酶切位点分析。方法 对酪氨酸、缬氨酸、天冬氨酸、天冬氨酸 (YVDD)及酪氨酸、异亮氨酸、天冬氨酸、天冬氨酸 (YIDD)变异病人进行测序分析。结果 在HBV感染的病例中 ,不仅检出酪氨酸、蛋氨酸、天冬氨酸、天冬氨酸 (YMDD)到YIDD /YVDD变异 ,还存在亮氨酸、亮氨酸、亮氨酸、亮氨酸 (LLLL)到亮氨酸、蛋氨酸、亮氨、亮氨酸 (LMLL)变异 ,天冬氨酸、亮氨酸、组氨酸、天冬氨酸 (DLHD)到天冬氨酸、蛋氨酸、组氨酸、天冬氨酸 (DMHD)变异及亮氨酸、亮氨酸、丙氨酸、谷氨酸 (LLAQ )到亮氨酸、蛋氨酸、丙氨酸、谷氨酸 (LMAQ)变异。酶切位点分析结果表明不同的变异株存在不同的限制性酶切位点。结论 提示这些变异位点均在“ATG”(M)蛋氨酸密码子尚需进一步探讨研究。YVDD、YIDD、LMAQ变异与拉米夫定治疗有关
Objective To establish rapid and specific detection of HBV gene mutation and restriction enzyme analysis of the mutant strains. Methods Tyrosine, valine, aspartic acid, aspartic acid (YVDD) and tyrosine, isoleucine, aspartic acid, aspartic acid (YIDD) mutation patients were sequenced. Results In cases of HBV infection, not only tyrosine, methionine, aspartic acid, aspartic acid (YMDD) to YIDD / YVDD mutations were detected, but also leucine, leucine, leucine, Leucine, Methionine, Leuconine, Leucine (LMLL) Variants, Aspartic, Leucine, Histidine, Aspartic Acid (DLHD) to Aspartic Acid, Methionine , Histidine, aspartate (DMHD) variation and leucine, leucine, alanine, glutamic acid (LLAQ) to leucine, methionine, alanine, glutamic acid (LMAQ) . Analysis of restriction enzyme sites showed that there are different restriction sites for different variants. The results suggest that all of these mutations are in the “ATG” (M) methionine codon needs further study. YVDD, YIDD, LMAQ mutation and lamivudine treatment