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目的探讨双特异性磷酸酶-6(DUSP-6)基因甲基化在鼻咽癌侵袭转移中的作用。方法应用甲基化特异性PCR和逆转录PCR分别检测38例鼻咽癌组织和14例慢性鼻咽炎组织中双特异性磷酸酶-6基因甲基化状态及其m RNA表达情况。结果 38例鼻咽癌组织中有29例发生双特异性磷酸酶-6基因甲基化,14例鼻咽部慢性炎症组织中无一例发生双特异性磷酸酶-6基因甲基化。DUSP-6基因甲基化在鼻咽癌各分期中差异无统计学意义(χ2=3.217,P=0.105>0.05),而在有淋巴结转移的鼻咽癌组织中DUSP-6基因甲基化(24/26)明显高于无淋巴结转移(5/12)的鼻咽癌组织,差异有统计学意义(χ2=13.216,P=0.000<0.05)。甲基化的鼻咽癌组织中双特异性磷酸酶-6弱或无表达,非甲基化的鼻咽癌组织和慢性鼻咽炎组织中双特异性磷酸酶-6强表达。结论鼻咽癌中双特异性磷酸酶-6表达与其基因甲基化状态有关,双特异性磷酸酶-6基因甲基化可能是鼻咽癌侵袭转移的重要因素。
Objective To investigate the role of methylation of bispecific phosphatase-6 (DUSP-6) gene in the invasion and metastasis of nasopharyngeal carcinoma. Methods The methylation status and m RNA expression of bispecific phosphatase-6 gene in 38 cases of nasopharyngeal carcinoma and 14 cases of chronic nasopharyngitis were detected by methylation-specific PCR and reverse transcription PCR respectively. Results The methylation of bispecific phosphatase - 6 gene was observed in 29 of 38 nasopharyngeal carcinoma tissues, and none of the 14 nasopharyngeal chronic inflammatory tissues showed methylation of bispecific phosphatase - 6 gene. The methylation of DUSP-6 gene in nasopharyngeal carcinoma had no statistical significance (χ2 = 3.217, P = 0.105> 0.05), while methylation of DUSP-6 gene in nasopharyngeal carcinoma with lymph node metastasis 24/26) were significantly higher than those without lymph node metastasis (5/12), the difference was statistically significant (χ2 = 13.216, P = 0.000 <0.05). Methylation of nasopharyngeal carcinoma in the double-specific phosphatase-6 weak or no expression, non-methylated nasopharyngeal carcinoma and chronic nasopharyngitis tissue specific expression of bispecific phosphatase-6. Conclusion The expression of bispecific phosphatase-6 in nasopharyngeal carcinoma is related to its methylation status. Methylation of bispecific phosphatase-6 gene may be an important factor in the invasion and metastasis of nasopharyngeal carcinoma.