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近十多年来,国内外广泛开展花培单倍体育种研究,已在许多作物中取得了成功.但在茶树花药培养方面的研究,至今未见诱导产生植株的报导.近年来,我们对此进行探索.供试品种为“福云6号”“福云7号”等,培养基采用Blaydes、Murashyge、Skoog、改良MS、N6、H、NH、SJ—1为基础,附加K、BA、2.4—D、NAA、GA、Ad、DC—2、CM、YE、谷氨酰胺、丝氨酸、半胱氨酸、石油助长剂等,蔗糖6～8%,脂0.8～1%,PH5.8～6.7.培养基分装于培养瓶后经15磅压力灭菌20分钟.接种花药的花粉发育期为单
In recent ten years, the research on haploid breeding of flower culture has been carried out extensively in China and abroad, and it has been successful in many crops. However, there has been no report on the induction of plant culture in tea plant anther so far. The test varieties were “Fuyun 6” and “Fuyun 7”. The medium was based on Blaydes, Murashyge, Skoog, modified MS, N6, H, NH and SJ-1 with K, BA , 2.4-D, NAA, GA, Ad, DC-2, CM, YE, glutamine, serine, cysteine, petroleum boosters and the like, sucrose 6-8%, fat 0.8-1%, pH 5.8 ~ 6.7 The culture medium was subdivided into flasks and sterilized by 15 pounds for 20 minutes.The pollen of inoculated anthers developed at a single