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为研究盐胁迫下微粒体膜Ca~(2+)-ATPase活性变化,试验采用营养液培养的方法,研究巴西蕉幼苗在60 mmol·L~(-1)的NaCl胁迫4、8、12、24和48 h下,增加外源CaCl_2和钙离子螯合剂EGTA处理,测定其叶片和根部分细胞膜上的Ca~(2+)-ATPase活性。结果表明,巴西蕉幼苗根和叶片的质膜、液泡膜及内质网膜的Ca~(2+)-ATPase活性各不同,且CaCl_2处理的Ca~(2+)-ATPase活性均显著高于其它3种处理。在NaCl胁迫4 h时,CaCl_2处理的Ca~(2+)-ATPase活性均显著升高,而EGTA处理的则显著下降。幼苗质膜和液泡膜的Ca~(2+)-ATPase活性远远大于内质网膜Ca~(2+)-ATPase。这表明了质膜和液泡膜Ca~(2+)-ATPase活性主要参与巴西蕉幼苗Ca~(2+)稳态的调控。
In order to study the changes of Ca ~ (2 +) - ATPase activity of microsomal membranes under salt stress, the nutrient solution culture was used to study the effects of NaCl stress of 4 mmol·L -1 NaCl solution on the growth of Brazilian banana seedlings. At 24 and 48 h, exogenous CaCl 2 and calcium ion chelator EGTA were added respectively to measure Ca 2+ (2 +) - ATPase activity in the plasma membrane of leaves and roots. The results showed that there were different Ca ~ (2 +) - ATPase activities in plasma membrane, vacuole membrane and endoplasmic reticulum in the roots and leaves of Brazilian banana plantlets, and the activities of Ca ~ (2 +) - ATPase in CaCl_2 treatment were significantly higher than those in CaCl2 Other three kinds of treatment. Ca (superscript 2 +) - ATPase activity of CaCl 2 treatment increased significantly at 4 h NaCl stress, but decreased significantly at EGTA treatment. The Ca ~ (2 +) - ATPase activity of plasma membrane and tonoplast in seedling was far greater than Ca ~ (2 +) - ATPase in endoplasmic reticulum. This indicated that Ca ~ (2 +) - ATPase activity of plasma membrane and tonoplast is mainly involved in the regulation of Ca ~ (2+) homeostasis in Brazilian banana seedlings.