论文部分内容阅读
目的 :观测人乳头瘤病毒DNA在舌癌及其他口腔非鳞状上皮细胞性肿瘤组织中的存在情况。方法 :收集 6 9例各种口腔及颌面部肿瘤组织 ,HE染色后常规病理学检测 ;提取肿瘤组织DNA ,以粘膜型HPV通用引物进行PCR扩增。结果 :4例 (4 / 9)舌癌 ,6例 (6 / 2 5 )腮腺混合瘤 ,2例 (2 / 9)颌骨肉瘤 ,2例 (2 / 15 )涎腺恶性肿瘤组织中HPVDNA呈阳性。而腺淋巴瘤、粘膜白斑、基底细胞腺瘤、颌骨造釉细胞瘤、腺泡细胞瘤组织中未发现有HPVDNA。结论 :部分口腔颌面部肿瘤可检测到HPVDNA ,HPV感染可能在上述肿瘤发生过程中起一定的作用。
Objective: To observe the existence of human papillomavirus DNA in tongue cancer and other oral non-squamous cell tumor tissues. Methods: Sixty-nine cases of oral and maxillofacial tumors were collected and stained with HE for routine pathological examination. Tumor DNA was extracted and amplified by mucosal HPV universal primers. Results: HPVDNA in 4 cases (4/9) of tongue cancer, 6 cases (6/25) parotid mixed tumor, 2 cases (2/9) of jaw osteosarcoma and 2 cases of salivary gland malignant tumor Positive. The adenolymphoma, leukoplakia, basal cell adenoma, jaw ameloblastoma, acinar cell tumor tissue was not found in HPVDNA. Conclusion: HPVDNA can be detected in some oral and maxillofacial tumors. HPV infection may play a role in the above tumorigenesis.