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目的：为探讨肿瘤坏死因子－α（ＴＮＦ－α）、一氧化氮（ＮＯ）、白介素２（ＩＬ－２）在非胰岛素依赖型糖尿病（ＮＩＤＤＭ）肾病发病机制中的作用。方法：选取ＮＩＤＤＭ患者３６例，采用酶联免疫吸附法、放射免疫检测法、比色法等方法检测ＮＩＤＤＭ患者血ＴＮＦ－α、ＮＯ、ＩＬ－２以及糖化血红蛋白（ＨｂＡ１Ｃ）、血浆尿素氮（ＢＵＮ）、血肌酐（Ｓｃｒ）等相关指标，并采用ｔ检验、直线相关等统计方法分析实验数据。结果：ＮＩＤＤＭ肾病患者的ＴＮＦ－α、ＮＯ水平均显著高于对照组及ＮＩＤＤＭ无并发症组，而ＩＬ－２则明显下降，其中ＮＩＤＤＭ肾病组中ＴＮＦ－α与ＨｂＡ１Ｃ呈正相关，ＩＬ－２水平与ＢＵＮ、Ｓｃｒ呈负相关；而ＮＯ与ＨｂＡ１Ｃ、ＢＵＮ、Ｓｃｒ均无显著相关关系。结论：本研究提示ＴＮＦ－α、ＮＯ水平升高是导致ＮＩＤＤＭ肾病发生的因素之一，而ＩＬ－２下降易继发感染，也可能加重肾功能障碍。
Objective: To investigate the role of tumor necrosis factor-α (TNF-α), nitric oxide (NO) and interleukin-2 (IL-2) in the pathogenesis of non-insulin dependent diabetes mellitus (NIDDM) Methods: Totally 36 patients with NIDDM were enrolled in this study. Enzyme-linked immunosorbent assay, radioimmunoassay and colorimetric assay were used to detect the levels of TNF-α, NO, IL-2 and HbA1C, BUN ), Serum creatinine (Scr) and other related indicators, and using t test, linear correlation and other statistical methods to analyze the experimental data. Results: The levels of TNF-α and NO in patients with NIDDM nephropathy were significantly higher than those in controls and NIDDM without complications, while the levels of IL-2 were significantly decreased. The levels of TNF-α and HbA1C in NIDDM nephropathy group were positively correlated with IL-2 And BUN, Scr was negatively correlated; NO and HbA1C, BUN, Scr no significant correlation. Conclusion: This study suggests that elevated TNF-α and NO levels are one of the factors leading to the development of NIDDM nephropathy. However, the decrease of IL-2 may be secondary to infection and may also aggravate renal dysfunction.