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目的探讨Wistar大鼠海马星形胶质细胞N-甲基-D-天冬氨酸受体(NMDAR)亚单位在β-淀粉样蛋白(Aβ)25～35毒性作用下的表达变化特点。方法大鼠海马原代培养细胞,加Aβ25～35(10μmol/L)分别作用1h、24h后,应用免疫荧光方法检测对照组和加药组中NR1、NR2A和NR2B的表达情况(n=10)。结果对照组海马星形胶质细胞表达NR1、NR2A和NR2B,阳性点状颗粒主要分布在细胞胞体和突起上,在胞体部位分布密集,在细胞突起则散在分布。经Aβ25～35作用后,三者表达均显著增强(P<0.05)。Aβ1h组和24h组相比,NR1无显著变化,NR2A、NR2B的表达随作用时间增加显著增强(P<0.05)。结论正常状态下海马星形胶质细胞可表达NMDAR亚单位(NR1、NR2A和NR2B);Aβ25～35作用会引起NMDAR各亚单位表达显著增强,NR1的表达变化与NR2A和NR2B的变化表现出不一致性。
Objective To investigate the changes of N-methyl-D-aspartate receptor (NMDAR) subunit of hippocampal astrocytes in Wistar rats induced by β-amyloid (Aβ) 25-35 toxicity. Methods The cultured primary cultured hippocampal cells of rats were treated with Aβ25-35 (10μmol / L) for 1h and 24h, respectively. The expression of NR1, NR2A and NR2B in control group and dosing group were detected by immunofluorescence (n = 10) . Results The hippocampal astrocytes expressed NR1, NR2A and NR2B in the control group. The positive dot-like particles were mainly distributed in the cell body and the protuberance, densely distributed in the cytoplasm, and scattered in the cell protuberances. The Aβ25 ~ 35 effect, the three were significantly enhanced (P <0.05). Compared with 24h group, there was no significant change of NR1 expression in Aβ1h group, and the expression of NR2A and NR2B increased significantly with the increase of treatment time (P <0.05). CONCLUSION: NMDAR subunits (NR1, NR2A and NR2B) can be expressed in hippocampal astrocytes under normal conditions. The effect of Aβ25-35 on the expression of NMDAR subunits is significantly enhanced. The changes of NR1 and NR2A and NR2B are not consistent Sex.