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目的了解潍坊市城区大气PM_(2.5)对小鼠外周血淋巴细胞的遗传毒性。方法收集2013年12月(采暖期)和2014年4月(非采暖期)潍坊市城区大气监测数据,包括SO_2、NO_2、PM_(10)、CO、O)3、PM_(2.5)和空气质量指数(AQI)。同时采集潍坊城区大气PM_(2.5)并制备成不同浓度的颗粒物悬液。将48只小鼠随机分为6组,每组8只,即阴性对照组(生理盐水)、阳性对照组(环磷酰胺)、采暖期高剂量组、采暖期低剂量组、非采暖期高剂量组、非采暖期低剂量组,采用气管滴注方式进行染毒,染毒容量为0.3 ml/kg(低剂量组:1.5 mg/kg;高剂量组:7.5 mg/kg),每日1次,连续染毒14 d。采用胞质分裂阻滞微核试验检测其细胞毒性及遗传损伤情况。结果采暖期大气SO)2、NO)2、CO、O)3、PM_(10)、PM_(2.5)和AQI的日平均值均高于非采暖期,差异均有统计学意义(P<0.05)。胞质分裂阻滞微核试验显示,对于相同采样期,采暖期高剂量组双核细胞率、核分裂指数低于低剂量组,坏死细胞率、微核率、核芽率高于低剂量组;非采暖期高剂量组坏死细胞率高于低剂量组,差异均有统计学意义(P<0.05)。对于相同剂量组,非采暖期低剂量组微核细胞率、核质桥率低于采暖期;非采暖期高剂量组双核细胞率、核分裂指数高于采暖期,坏死细胞率、微核细胞率、微核率、核质桥率、核芽率低于采暖期,差异均有统计学意义(P<0.05)。结论采暖期PM_(2.5)可使小鼠外周血淋巴细胞微核率增加,并有较强的细胞毒性。
Objective To understand the genotoxicity of PM_ (2.5) in peripheral blood lymphocytes of mice in urban area of Weifang City. Methods The urban atmospheric monitoring data of Weifang city in December 2013 (heating period) and April 2014 (non-heating period) were collected, including SO_2, NO_2, PM_ (10), CO_2O, PM_ (2.5) and air quality Index (AQI). At the same time, Weifang city atmosphere PM_ (2.5) was collected and prepared into different concentrations of particulate matter suspension. Forty-eight mice were randomly divided into 6 groups with 8 rats in each group, namely negative control group (normal saline), positive control group (cyclophosphamide), high-dose heating group, low-dose heating group, Dose group, low-dose non-heating group, the use of tracheal instillation manner, exposure capacity of 0.3 ml / kg (low dose group: 1.5 mg / kg; high dose group: 7.5 mg / kg) Times, continuous exposure to 14 d. Cytotoxicity and genetic damage were detected by cytokinesis-block micronucleus test. Results The average daily values of SO 2, NO 2, CO, O 3, PM 10, PM 2.5 and AQI in heating period were all higher than those in non-heating period (P <0.05 ). Cytokinesis in micronuclei test showed that for the same sampling period, the rates of nucleated cell nucleus and mitotic index in high-dose heating group were lower than those in low-dose group, the rate of necrotic cells, micronucleus rate and nuclear bud rate were higher than those in low dose group; In the high-dose heating group, the necrotic cell rate was higher than that in the low-dose group, the difference was statistically significant (P <0.05). For the same dose group, the rates of micronuclei and nucleus were lower in non-heating low-dose group than in heating group. The rates of dual-nucleus and mitotic index in high-dose non-heating group were higher than those in heating period, necrosis and micronuclei , Micronucleus rate, nuclear cybrid bridge rate, nuclear bud rate lower than the heating period, the differences were statistically significant (P <0.05). Conclusion PM_ (2.5) in heating period can increase the micronucleus rate of peripheral blood lymphocytes in mice and has strong cytotoxicity.