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目的研究两种检测方法测得二氧化氯含量与其杀菌效果之间的关系,以评价测定方法的准确性。方法采用丙二酸碘量法与五步碘量法测定二氧化氯含量,采用悬液定量杀菌试验方法进行杀菌效果评价。结果以丙二酸碘量法测定为准,在4 d之内,随放置时间的延长,补加二氧化氯至初始浓度对金黄色葡萄球菌、黑曲霉和枯草杆菌黑色变种芽孢的杀灭效果均呈下降趋势。以五步碘量法测定为准,在第4 d,补加二氧化氯至初始浓度,其对上述细菌的杀菌效果与初始浓度时无明显差别。结论用五步碘量法测得的二氧化氯浓度与杀菌效果之间呈对应关系,对二氧化氯的连续使用具有指导意义;而用丙二酸碘量法测得的含量与其杀菌效果不对应。
Objective To study the relationship between chlorine dioxide content and bactericidal effect measured by the two methods to evaluate the accuracy of the method. Methods The content of chlorine dioxide was determined by the method of iodine malonate and the five-step iodine method. The bactericidal effect was evaluated by the method of suspension quantitative sterilization. The results were based on the determination of malonic acid iodine, within 4 days, with the extension of storage time, additional chlorine dioxide to the initial concentration of Staphylococcus aureus, Aspergillus niger and Bacillus subtilis killing effect All showed a downward trend. Based on the five-step iodometric method, no additional difference was found between the bactericidal effect and the initial concentration of chlorine dioxide added to the initial concentration on the 4th day. Conclusions The chlorine dioxide concentration measured by the five-step iodometric method has a corresponding relationship with the bactericidal effect, which is instructive for the continuous use of chlorine dioxide. However, the content measured by the iodometric method of malondialdehyde and the bactericidal effect are not correspond.