采用引物步移法测得白边大叶蝉Kolla paulula(Walker)线粒体基因组90%左右的序列,并分析了该叶蝉的线粒体基因组特征。基于23个物种(半翅目)的蛋白编码基因序列,以最大似然法和贝叶斯法构建了头喙亚目系统发育树。已测得序列长度为13 579 bp(AT:73.33%),其中包含了13个蛋白编码基因、21个t RNA基因和1个r RNA基因。除了ND5基因使用GTT作为起始密码子外,其他所有蛋白编码基因均使用ATN作为起始密码子;除了CO II使用不完整的T作为终止密码子,其他所有蛋白编码基因均使用TAA或TAG作为终止密码子。21个t RNA基因中,除了t RNASer(AGN)缺失1个稳定的茎环结构外,其他所有t RNA基因均能形成典型的三叶草二级结构。系统发育分析结果表明,进化树明显可以被分为沫蝉总科+(角蝉总科+蜡蝉总科);白边大叶蝉属于角蝉总科的叶蝉科,白边大叶蝉线粒体基因组特征与其他叶蝉科昆虫相同。 The mitochondrial genome sequence of about 90% of the white-leafhopper, Kolla paulula (Walker), was measured by primer-walking method and the mitochondrial genome of the leafhopper was analyzed. Based on the sequence of protein coding genes of 23 species (Hemiptera), the head-beaked suborder phylogenetic tree was constructed using the maximum likelihood method and Bayesian method. The measured sequence length was 13 579 bp (AT: 73.33%), which contained 13 protein-coding genes, 21 tRNA genes and 1 rRNA gene. All of the other protein-coding genes used ATN as a start codon, except for the ND5 gene which used GTT as a start codon. All of the other protein-coding genes used TAA or TAG as the stop codon except that CO II used incomplete T as a stop codon Stop codon Among the 21 tRNA genes, all of the tRNA genes formed a typical cloverleaf secondary structure, except that tRNASer (AGN) lacked a stable stem-loop structure. The results of phylogenetic analysis showed that the phylogenetic tree can be divided into two subfamilies: S. cicadaceae + (C. cicadaceae + C. cicada) Mitochondrial genome features the same as other Cicadellidae.