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发根农杆菌(Agrobacterium rhizogenes)Ri质粒做为基因载体的特点是:(1)由于Ri质粒的T—DNA不影响植物的再生,可直接用做中间载体的受体;(2)Ri质粒的T—DNA插入受体细胞基因组DNA诱发的毛状根,起源于单细胞,所以由毛状根产生的再生植株是纯合的;(3)转化的毛状根易检测与再生植株。因此,近年来利用Ri质粒做转基因的载体,向受体植物导入外源基因的研究日益受到重视。
The characteristics of Ri plasmid of Agrobacterium rhizogenes as a gene carrier are as follows: (1) Since the T-DNA of Ri plasmid does not affect plant regeneration, it can be directly used as an intermediate carrier; (2) T-DNA is inserted into the hairy roots induced by the genomic DNA of recipient cells and originates from a single cell. Therefore, the regenerated plant derived from the hairy root is homozygous; (3) Transformed hairy roots are easily detected and regenerated. Therefore, in recent years, the use of Ri plasmid as a transgene carrier, the introduction of foreign genes into the recipient plant research has received increasing attention.