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本观察是根据得自胞蚴的20个后前期细胞和中期细胞的染色体分析。曼氏血吸虫来源于波多黎各。染色体系由4只阳性扁卷螺Biomphalaria glabrata中未知性别的胞蚴制备而成的。活的螺蛳用秋水仙碱处理后,取出含有血吸虫幼虫的消化腺,用配有19号针头的注射器在软体动物生理盐水(无Ca~(++)和Mg~(++))中研碎成悬液。悬液用低渗的软体动物生理盐水(稀释9:1)处理约30分钟,然后离心,并用甲醇醋酸(3:1)固定,再经离心
This observation is based on the chromosomal analysis of 20 pre-procellular and metaphase cells obtained from the cytomegalovirus. Schistosoma mansoni originates in Puerto Rico. The chromosomal system was prepared from an unidentified sexually transmitted metazoan of 4 positive Bombyx mori Biomphalaria glabrata. After the live snail was treated with colchicine, the digestive gland containing the larvae of Schistosoma japonicum was removed and pulverized in a mollusc physiological saline (without Ca ++ and Mg ++) using a syringe equipped with a 19 gauge needle Suspension. The suspension is treated with hypotonic saline bath (diluted 9: 1) for about 30 minutes, then centrifuged and fixed with methanol-acetic acid (3: 1) and centrifuged