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复方氨基酸注射液的色氨酸含量,因活性炭等的吸附,是生产过程中严格控制的项目。而应用氨基酸分析仪或比色法检测,尚不能适应生产需要。本文应用紫外分光光度法测定色氨酸含量,在生产控制中获得较满意的效果。现报告如下: 一、仪器药品和试剂 751G紫外分光光度计上海分析仪器厂出品 100ml容量瓶 1ml移液管复方氨基酸注射液(lls)及中间体本厂出品色氨酸对照品日本味之素出品 0.1mol/L氢氧化钠液
Compound amino acid injection of tryptophan content, due to the adsorption of activated carbon, is the production process strictly controlled projects. The application of amino acid analyzer or colorimetric detection, still can not meet the production needs. In this paper, the determination of tryptophan content by UV spectrophotometry in the production control to obtain more satisfactory results. Now report as follows: First, the instruments and reagents 751G UV spectrophotometer Shanghai Analytical Instrument Factory produced 100ml volumetric flask 1ml pipette compound amino acid injection (lls) and intermediates factory production of tryptophan reference substance Ajinomoto produced 0.1mol / L sodium hydroxide solution