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目的:探讨宫颈癌患者血清中人乳头瘤状病毒(HPV)DNA和宫颈内病灶组织HPV DNA二者的相关性和宫颈癌患者血清中HPV DNA检测的意义。方法:对确诊为宫颈癌的93例患者采集病灶组织和静脉血,采用PCR方法对所有的采集标本进行HPV DNA和HPV亚型的检测。结果:宫颈癌患者病灶组织中HPV阳性率为64.52%(60/93),以高危型HPV16、18和HPV58亚型为主;在血清检测中HPV阳性率为49.46%(45/93),两种检测方法 HPV阳性率差异有统计学意义(P<0.05)。在两种检测方法中,高危型HPV16、18亚型相同病例数为23例,高危型HPV16、58亚型相同病例数为15例,两种检测方法的高危型HPV16、18、58亚型检测差异无统计学意义(P>0.05);其他HPV阳性亚型均不相同。在血清检测7例HPV阳性患者其病灶组织HPV检测呈阴性。结论:单纯检测患者血清中HPV感染不能作为临床诊断宫颈癌的肿瘤标志物,而血清中高危险型HPV16、18和58亚型检测对宫颈癌的确诊有辅助价值。
Objective: To investigate the correlation between the HPV DNA in the serum of patients with cervical cancer and HPV DNA in cervical intracervical tissue and the detection of HPV DNA in the serum of patients with cervical cancer. Methods: Tissues and venous blood samples were collected from 93 patients diagnosed as cervical cancer. HPV DNA and HPV subtypes were detected by PCR in all collected specimens. Results: The positive rate of HPV in lesions of cervical cancer patients was 64.52% (60/93), with high-risk HPV16,18 and HPV58 subtypes as the major ones. The positive rate of HPV in serum was 49.46% (45/93) The detection rate of HPV positive rate was statistically significant (P <0.05). Among the two detection methods, the same number of high-risk HPV16,18 subtypes was 23 cases, high-risk HPV16,58 same case number 15 cases, two detection methods of high-risk HPV16,18,58 subtype detection The difference was not statistically significant (P> 0.05); other HPV-positive subtypes are not the same. Seven cases of HPV-positive patients in the serum detection of HPV in the lesion tissue was negative. Conclusion: The detection of HPV infection in the serum of patients can not be used as the tumor marker for clinical diagnosis of cervical cancer. However, the detection of high-risk HPV16, 18 and 58 subtypes in serum is valuable for the diagnosis of cervical cancer.