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【目的】为开发高效的高浓度木质纤维素燃料乙醇蒸馏废水厌氧处理及资源化利用工艺,以活性炭为载体,在实验室规模上对高温厌氧流化床反应器处理木质纤维素燃料乙醇蒸馏废水进行研究。【方法】反应器经65 d梯度驯化后启动,对工艺参数进行一系列优化,并通过基于16S rRNA基因的分子生态学技术分析厌氧污泥中的优势菌群。【结果】实验获得了最优的反应条件和处理效果:厌氧流化床反应器(Anaerobic fluidized bed reactor,AFBR)在温度55±1°C、有机负荷率(OLR)13.8 g COD/(L·d)及水力停留时间(HRT)48 h操作时,COD去除率达到90%以上,同时甲烷产率达到290 mL/g COD;菌群鉴定分析结果显示高温厌氧活性污泥中Clostridia所占比例最大,产甲烷菌属以Methanoculleus和Methanosarcina为主,其它功能菌群主要为Alphaproteobacteria等。【结论】AFBR反应器可高效降解木质纤维素燃料乙醇蒸馏废水并产生生物能源甲烷,其反应体系内微生物种类丰富。
【Objective】 In order to develop an efficient and efficient anaerobic treatment and resource utilization technology of high concentration lignocellulose fuel ethanol distillation wastewater, activated carbon was used as a carrier to treat high temperature anaerobic fluidized bed reactor on lignocellulose fuel ethanol Distilled wastewater was studied. 【Method】 The reactor was acclimated after a 65-day gradient acclimation. A series of optimization of process parameters were carried out. The dominant bacteria in anaerobic sludge were analyzed by molecular ecology based on 16S rRNA gene. 【Result】 The optimal reaction conditions and treatment effects were obtained in this experiment: Anaerobic fluidized bed reactor (AFBR) was used at a temperature of 55 ± 1 ° C with an OLR of 13.8 g COD / (L · D) and hydraulic retention time (HRT) 48 h, the removal rate of COD was above 90% and the methane yield reached 290 mL / g COD. The results of microbial community identification showed that Clostridia was occupied by high temperature anaerobic activated sludge Methanoculleus and Methanosarcina are the main genera of methanogens, while Alphaproteobacteria and others are the major functional groups. 【Conclusion】 The AFBR reactor can efficiently degrade ethanol wastewater from lignocellulose fuel and produce methane, a bioenergy source, with abundant microbial species in the reaction system.