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目的：探讨口腔链球菌基因分型，比较细菌相互间基因差异。方法：设计２对１０碱基随意引物，提取变链球菌、血链球菌染色体ＤＮＡ，分别用一对引物进行ＰＣＲ扩增。结果：变链球菌、血链球菌扩增的ＤＮＡ基因片段有较大区别。设计不同序列引物，扩增的ＤＮＡ片段亦不同，可以分辨不同的基因位点。结论：随意引物ＰＣＲ检测方法稳定，可作为细菌基因分型较好的方法
Objective: To investigate the genotyping of Streptococcus oralis and to compare the genetic differences among bacteria. Methods: Two pairs of random primers of 10 bases were designed and the chromosomal DNA of Streptococcus mutans and Streptococcus sanguis were extracted. A pair of primers were used for PCR amplification. Results: Streptococcus mutans and Streptococcus sanguis amplified DNA gene fragments are quite different. Different sequences of primers are designed to amplify the DNA fragments are also different, you can distinguish different gene loci. Conclusion: The random primer PCR method is stable and can be used as a good method for bacterial genotyping