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目的:建立痛经宝颗粒中原儿茶醛的含量测定方法。方法:色谱柱:Shimadzu C_(18)柱(250 mm×4.6 mm,5μm);以甲醇-0.4%冰醋酸(12:88)为流动相;流速:1.0 ml·min~(-1);检测波长为280 nm。结果:原儿茶醛在6.2～62.0μg·ml~(-1)范围内呈良好的线性关系(r=0.999 6),平均回收率为99.4%,RSD为1.07%。结论:本法结果准确度高,重复性好,可作为痛经宝颗粒的质量评价依据。
Objective: To establish a method for the determination of protocatechuic aldehyde in Tongjingbao granule. METHODS: The chromatographic column was Shimadzu C_ (18) column (250 mm × 4.6 mm, 5 μm) with methanol-0.4% glacial acetic acid (12:88) as mobile phase. The flow rate was 1.0 ml · min -1. The wavelength is 280 nm. RESULTS: Protocatechuic aldehyde showed a good linearity (r = 0.999 6) in the range of 6.2 ~ 62.0μg · ml ~ (-1) with an average recovery of 99.4% and a RSD of 1.07%. Conclusion: This method has the advantages of high accuracy and good repeatability, which can be used as the basis for quality evaluation of Tongjingbao granules.