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为探究干旱和增强UV-B辐射对3种胡枝子(美丽胡枝子、二色胡枝子、牛枝子)抗氧化酶活性和渗透调节物质的影响,采用盆栽试验,研究了正常生长(CK)、干旱胁迫(D)、增强UV-B辐射(UV-B)及二者复合胁迫(D+UV-B)下各植物叶片中抗氧化酶活性和渗透调节物质的动态响应.结果表明:3种处理下各植物叶片中SOD活性较对照值均呈先下降后上升趋势,且与单因子处理相比,牛枝子和二色胡枝子在复合胁迫下其SOD活性更高;增强UV-B辐射和复合处理下POD活性随胁迫时间总体呈先上升后下降趋势;CAT活性在干旱和复合胁迫下高于对照值,而在增强UV-B辐射下较对照值有所下降.3种植物叶片渗透调节物质含量(可溶性蛋白、可溶性糖、游离脯氨酸)在各处理中较对照值均有所增加,并呈先上升后下降趋势,但种间差异显著.3种处理均导致各植物叶片内MDA含量不同程度的升高,升高幅度的大小依次为牛枝子>美丽胡枝子>二色胡枝子.通过隶属函数法综合评价得出,复合处理下二色胡枝子和美丽胡枝子具有较强的抗氧化能力,牛枝子抗氧化能力较弱.综上,与干旱胁迫和增强UV-B辐射相比,复合胁迫对植物的伤害程度更小,说明干旱与增强UV-B辐射可以相互缓减彼此对植物的伤害,且美丽胡枝子和二色胡枝子在抵抗干旱胁迫和增强UV-B辐射方面具有一定优势.
In order to explore the effects of drought and UV-B radiation on the activities of antioxidant enzymes and osmolytes of three species of Lespedeza (Lespedeza, Lespedeza, Lespedeza, Amaranthus), pot experiments were conducted to study the effects of normal growth (CK) The dynamic responses of antioxidant enzymes and osmolytes in leaves of all plants under drought stress (D), enhanced UV-B radiation (UV-B) and their combination stress (D + UV-B) Compared with the single-factor treatment, the activities of SOD in the leaves of all the plants under the treatment of compound stress were higher than those of the control. Under the combined stress, the activity of SOD was increased. The UV-B radiation and The activities of POD increased firstly and then decreased with the increase of stress time under the combined treatment. CAT activity was higher than the control under drought and combined stress, but decreased under the enhanced UV-B radiation. The osmotic adjustment of three plants The contents of soluble protein, soluble sugar and free proline increased in each treatment compared with the control value, and then increased firstly and then decreased, but the interspecies difference was significant.The three treatments led to the change of MDA Different levels of increase, the size of the increase in size The results showed that under the composite treatment, the Lespedeza bicolor and the Lespedeza macrocarpa had strong anti-oxidation ability, and the antioxidant capacity of the Lespedeza bicolor was weak. , Compared with drought stress and enhanced UV-B radiation, the composite stress damage to plants to a lesser degree, indicating that drought and enhanced UV-B radiation can mutually reduce each other on the plant damage, and Lespedeza bicolor and dichroa Branches have some advantages in resisting drought stress and enhancing UV-B radiation.