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１次灌胃给予土王峒酒，测得小鼠ＬＤ５０为２３．６０ｇ／ｋｇ，对小白鼠长期灌胃给予土王峒酒，能提高小鼠的耐缺氧、抗疲劳和耐寒能力，与对照组比较有非常显著性差异（Ｐ＜０．０１）。对大白鼠的睾酮实验结果表明，高、低剂量的土王峒酒长期给予，对大白鼠的雄性激素有明显的升高作用（Ｐ＜０．０１）。
The LD50 of the mice was determined to be 23.60g/kg by gavage for 1 time. The long-term intragastric administration of Tuwangfujiu to mice can improve the ability of mice to bear hypoxia, anti-fatigue and cold-resistance. There was a very significant difference in the control group (P<0.01). The results of testosterone test in rats showed that the long-term administration of high- and low-dose Tuwangfen had a significant increase in male hormones in rats (P<0.01).