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利用分子印迹技术,以马来松香丙烯酸乙二醇酯为交联剂,丙烯酸为功能单体研制了测定茶碱的新型电化学传感器.以自由基热聚合的方式于玻碳电极表面制备茶碱分子印迹聚合物膜.通过循环伏安法、电化学交流阻抗法及计时电流法研究了传感器对茶碱的响应特性.结果表明,在最佳的实验条件下,传感器的峰电流与茶碱浓度在2.00×10-7～3.45×10-4mol/L范围内呈现出良好的线性关系(线性相关系数为0.9961),检出限(S/N=3)为1.00×10-7mol/L;该传感器的选择性高,稳定性和重现性好,将此传感器用于茶碱缓释片中茶碱的含量测定,回收率为95.6%～103.8%.
A new electrochemical sensor for the theophylline was developed by using molecularly imprinted polymer with maleic rosin as cross-linking agent and acrylic acid as functional monomer. Theophylline was prepared on the surface of glassy carbon electrode by free radical thermal polymerization The molecularly imprinted polymer films were characterized by cyclic voltammetry, electrochemical impedance spectroscopy and chronoamperometry.The results showed that under the optimal experimental conditions, the peak current of the sensor and theophylline concentration The results showed that the detection limit (S / N = 3) was 1.00 × 10-7mol / L in the range of 2.00 × 10-7 ~ 3.45 × 10-4mol / L with a linear correlation coefficient of 0.9961. The sensor has high selectivity, good stability and reproducibility. The sensor was used in the theophylline content of theophylline sustained-release tablets, the recovery was 95.6% ~ 103.8%.