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目的:探讨不同浓度富血小板血浆(Platelet-rich plasma,PRP)对根尖乳头干细胞(Stem cell from the apical papilla,SCAP)增殖及矿化的影响。方法:两步离心法制备PRP,通过ELISA的方法测定PRP中血小板源性生长因子(PDGF-AB)和转化生长因子(TGF-β1)的浓度;CCK-8法、ALP活性检测法观察5%、10%、15%、20%PRP与对照组在1 d、2 d、3 d、4 d、5 d、6 d时对根尖乳头干细胞增殖及矿化作用的影响。结果:PRP中血小板浓度大于全血中血小板浓度,为全血中的5倍以上。CCK-8法测定不同浓度组的PRP对根尖乳头细胞增殖均有促进作用,以10%PRP增殖作用显着,P<0.05。PRP组与对照组相比,3 d时,ALP活性无显著性差异(P>0.05);6 d时,差异有显著性(P<0.05)。结论:PRP对根尖乳头干细胞的增殖及矿化均具有促进作用,以10%PRP增殖效应最为显著。
Objective: To investigate the effects of different concentrations of platelet-rich plasma (PRP) on the proliferation and mineralization of apical papillary stem cells (SCAP). Methods: Two-step centrifugation was used to prepare PRP. The concentrations of platelet-derived growth factor (PDGF-AB) and transforming growth factor (TGF-β1) in PRP were measured by ELISA. Effects of PRP and control group on the proliferation and mineralization of apical papillary stem cells in 1, 2, 3, 4, 5 and 6 days of PRP and 10%, 15% and 20% PRP groups. Results: The platelet concentration in PRP was greater than the platelet concentration in whole blood, which was more than 5 times of that in whole blood. CCK-8 assay of different concentrations of PRP on the proliferation of apex papillary cells were promoted role in the proliferation of 10% PRP significantly (P <0.05). There was no significant difference in ALP activity between PRP group and control group at 3 d (P> 0.05), but there was significant difference at 6 d (P <0.05). Conclusion: PRP can promote the proliferation and mineralization of apical papillary stem cells, and the most obvious effect is the proliferation of 10% PRP.