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目的比较葛根素磷脂复合物与葛根素原料药在家兔体内的生物利用度。方法家兔16只,随机分为两组,分别灌胃给与葛根素和葛根素磷脂复合物,用反相高效液相(RP-HPLC)法测定不同时间血浆中葛根素的浓度,采用3P97程序计算药代动力学参数。结果家兔分别灌胃给与葛根素及其磷脂复合物(50mg/kg,以葛根素计),葛根素的药-时过程符合开放型二室一级动力学模型,葛根素内服的AUC、Cmax、tpeak分别为(93.331±0.973)μg/(min·ml)、(0.693±0.011)μg/ml、(29.808±1.220)min,而葛根素磷脂复合物的AUC、Cmax、tpeak分别为(160.128±5.917)μg/(min·ml)、(0.816±0.017)μg/ml、(52.956±0.148)min,统计结果表明:AUC之间差异有显著性。结论磷脂可显著提高葛根素在家兔体内的生物利用度。
Objective To compare the bioavailability of puerarin phospholipid and puerarin in rabbits. Methods Twenty-six rabbits were randomly divided into two groups. The puerarin and puerarin complexes were administered intragastrically. The concentration of puerarin in plasma was determined by reverse-phase high performance liquid chromatography (RP-HPLC) The program calculates the pharmacokinetic parameters. Results The puerarin and its phospholipid complex (50mg / kg, puerarin) were administered intragastrically to puerarin. The puerarin-time course was in accordance with the open two-compartment first-order kinetic model. The AUC, Cmax and tpeak were (93.331 ± 0.973) μg / (min · ml), (0.693 ± 0.011) μg / ml and (29.808 ± 1.220) min respectively, while the AUC, Cmax and tpeak of the puerarin phospholipid complex were (160.128 ± 5.917) μg / (min · ml), (0.816 ± 0.017) μg / ml, (52.956 ± 0.148) min respectively. The statistical results showed that there was a significant difference between AUC. Conclusion Phospholipid can significantly increase the bioavailability of puerarin in rabbits.