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首次在国内报告小鼠前庭迷路的体外发育。采用周祥宁和Van De Water建立的HEMA水凝胶器官培养法培养第12～13天CBA/C57小鼠胚胎的内耳原基7～8天。内耳原基的上部发育成3个半规管、椭圆囊和球囊。半规管的壶腹嵴由一排感觉毛细胞和2～3排支持细胞构成。嵴的上方有正在发育的嵴顶。椭圆囊斑和球囊斑的表面都有一层耳石膜。这些囊斑内可见一层感觉毛细胞和1～2层支持细胞。超微结构研究发现壶腹嵴、椭圆囊斑和球囊斑的毛细胞有排列规则的静纤毛和表皮板,毛细胞的上部有丰富的线粒体,还可见内质网和高尔基体等细胞器。内耳体外培养可用以研究前庭迷路的发育机理、耳毒性药物、放射线等致病因素对前庭迷路的损害以及前庭迷路毛细胞的再生,对研究前庭迷路疾病引起的眩晕病的预防和治疗有积极作用。
For the first time in the domestic report mouse vestibular labyrinth in vitro development. The inner ear primordia of CBA / C57 mouse embryos from days 12 to 13 were cultured for 7-8 days using the HEMA hydrogel organ culture established by Zhou Xiangning and Van De Water. The upper part of the inner ear primordium developed into three semicircular canal, oval capsule and balloon. Semi-regulatory ampulla ossicles by a row of sensory hair cells and 2 to 3 rows of supporting cells. Above the crest is a developing crest. Oval bursa and balloon plaque surface has a layer of otolith. Within these cysts there is a layer of sensory hair cells and 1-2 layers of supporting cells. Ultrastructure studies found that ampulla, oval and balloon plaques hair cells arranged in regular cilia and epidermal plate, the upper part of the hair cells are rich in mitochondria, but also the endoplasmic reticulum and Golgi apparatus and other organelles. Internal ear culture can be used to study the development mechanism of vestibular labyrinth, ototoxic drugs, radiation and other pathogenic factors on the damage of vestibular labyrinth and regeneration of vestibular lost hair cells, to study the prevention and treatment of vertigo caused by vestibular labyrinth disease have a positive effect .