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目的：探讨人血中ＣＤ３＋ＣＤ１６－及ＣＤ３－ＣＤ１６＋细胞在异种肾移植（猪／人）间接体内模型中的变化。方法：利用模拟人体生理条件的体外灌注系统，将人新鲜血灌注离体猪肾２２只（实验组），人肾８只（对照组）。经免疫荧光双标记，用流式细胞仪检测灌注前及灌注后不同时间内，人血中ＣＤ３＋ＣＤ１６－及ＣＤ３－ＣＤ１６＋细胞的变化。结果：最初经由猪肾灌注出来的人血中ＣＤ３＋ＣＤ１６－细胞、ＣＤ３－ＣＤ１６＋细胞与灌注前相比，分别由（６７．８±３．４）％降至（３２．４±４．１）％和（１７．３±１．８）％降至（６．９±１．２）％。随着灌注时间的延长，两种细胞减少的趋势逐渐减弱；而经由人肾灌注的人血与灌注前相比，两种细胞无明显变化。结论：人血中Ｔ细胞、ＮＫ细胞可能通过与猪肾血管内皮细胞的粘附，参与了异种肾移植的排斥反应。
Objective: To investigate the changes of CD3 + CD16- and CD3-CD16 + cells in human blood in an indirect in vivo model of xenotransplantation (pig / human). Methods: In vitro perfusion system simulating human physiological conditions was used to instill fresh blood into 22 porcine kidney (experimental group) and 8 human renal (control group). The changes of CD3 + CD16- and CD3-CD16 + cells in human blood before and after perfusion were detected by immunofluorescence double labeling and flow cytometry. Results: Compared with those before perfusion, CD3 + CD16- and CD3-CD16 + cells in human blood were decreased from (67.8 ± 3.4)% to (32.4 ± 4.1)% And (17.3 ± 1.8)% to (6.9 ± 1.2)%, respectively. With the prolongation of perfusion time, the tendency of both cells decreased gradually; however, there was no obvious change in the two kinds of cells compared with those before perfusion. CONCLUSION: T cells and NK cells in human blood are involved in the rejection of xenotransplantation renal allografts via adhesion with porcine renal vascular endothelial cells.