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目的:建立气相色谱法测定香榧假种皮挥发油中α-蒎烯、β-蒎烯和柠檬烯含量的方法。方法:采用HP-1石英毛细管柱(0.25μm×320.00μm×29.9 m),进样口温度230℃,FID检测器,检测器温度250℃,柱温程序升温(起始温度50℃,保持2 min,8℃.min-1升温至130℃,保持3 min),载气N2,流速1.2 mL·min-1,进样方式为分流进样,分流比30∶1,进样量1μL,萘为内标物。结果:α-蒎烯、β-蒎烯和柠檬烯线性范围分别为0.070～2.230(r=1.000 0),0.075～2.393(r=1.000 0),0.070～2.240(r=0.999 9)g·L-1,平均回收率分别为103.0%(RSD 1.4%),99.1%(RSD 1.2%),97.7%(RSD 1.6%)。结论:建立的测定方法简便快速、准确,可为香榧假种皮挥发油质量控制提供参考。
OBJECTIVE: To establish a method for the determination of α-pinene, β-pinene and limonene in the essential oil of Emuia pseudoacacia by gas chromatography. Methods: HP-1 quartz capillary column (0.25 μm × 320 μm and 29.9 μm) was used. The inlet temperature was 230 ℃ and the FID detector was used. The temperature of the detector was 250 ℃. The temperature of the column was increased by 50 ℃. min, 8 ℃ .min-1 temperature to 130 ℃ for 3 min), carrier gas N2, flow rate of 1.2 mL · min-1, injection mode shunt injection, split ratio 30:1, injection volume 1μL, naphthalene As internal standard. RESULTS: The linear ranges of α-pinene, β-pinene and limonene were 0.070-2.230 (r = 1.000 0), 0.075-2.393 (r = 1.000 0), 0.070-2.240 (r = 0.999 9) g · L- 1, the average recoveries were 103.0% (RSD 1.4%), 99.1% (RSD 1.2%) and 97.7% (RSD 1.6%), respectively. Conclusion: The established determination method is simple, rapid and accurate, which can provide reference for the quality control of volatile oil from the seed coat of.