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目的:观察大豆磷脂酰胆碱对幼年大鼠胸段脊髓前角内突触数量和超微结构的影响,探讨大鼠脊髓神经突触可塑性及大豆磷脂酰胆碱作用机制。方法:①实验于2004-03/12在兰州大学基础医学院神经研究室完成。选用5周龄健康雄性Wistar大鼠22只。随机将大鼠分为2组:大豆磷脂酰胆碱添加喂养组和对照组,每组11只。大豆磷脂酰胆碱添加喂养组:大豆磷脂酰胆碱500mg/(kg·d),溶于50mL双蒸水灌胃,1次/d;对照组:等量双蒸水灌胃,1次/d。两组均自由进食(标准基础饲料)和普通饮水。②喂养8周,灌注取材,用透射电镜观察两组大鼠胸段脊髓灰质前角内突触数密度和突触活性区膜面积密度的变化。用点阵测试系统和点计数法测算脊髓胸段前角灰质内突触的数密度和突触活性区的膜面积密度。③计量资料差异比较采用t检验。结果:大鼠22只均进入结果分析。大豆磷脂酰胆碱添加喂养组大鼠胸段脊髓前角灰质内突触的数密度和突触活性区的膜面积密度均明显高于对照组[(38.27±3.98)个/μm3,(0.0587±0.007)μm2/μm3;(18.36±3.14)个/μm3,(0.0264±0.009)μm2/μm3,t=13.032,9.181,P<0.01]。结论:添加大豆磷脂酰胆碱喂养幼年大鼠,大鼠脊髓突触数量和突触活性区膜面积增加,提高了幼年大鼠脊髓神经突触可塑性。
OBJECTIVE: To observe the effect of soybean phosphatidylcholine on synaptic number and ultrastructure in anteroposterior angle of thoracic spinal cord in juvenile rats, and to explore the synaptic plasticity of soybean spinal cord and the mechanism of action of soybean phosphatidylcholine. METHODS: 1 The experiment was performed at the Department of Neurology, Lanzhou University School of Medicine from March to December 2004. Twenty-two healthy 5-week-old male Wistar rats were used. Rats were randomly divided into 2 groups: soybean phosphatidylcholine added to the feeding group and the control group, 11 in each group. Soybean phosphatidylcholine added to the feeding group: Soybean phosphatidylcholine 500mg/(kg·d), dissolved in 50mL double distilled water, once a day; control group: double distilled water, gavage, once/ d. Both groups eat freely (standard basal feed) and regular drinking water. 2 After feeding for 8 weeks, the rats were perfused and the transmission electron microscopy was used to observe the changes of synapse number density and synaptic membrane area density in the anterior horn of the thoracic spinal cord of the two groups. The number density of synapses in the thoracic anterior horn of the spinal cord and the membrane area density in the synaptic active area were calculated using a dot-matrix test system and dot counting. 3 Measurement data differences are compared using the t-test. RESULTS: Twenty-two rats were involved in the result analysis. The number density of synapses in the thoracic spinal anterior horn and the membrane area density of the synaptic active area in the phosphatidylcholine-added group were significantly higher than those in the control group [(38.27±3.98)/μm3, (0.0587±) 0.007) μm2/μm3; (18.36±3.14)/μm3, (0.0264±0.009) μm2/μm3, t=13.032, 9.181, P<0.01]. CONCLUSION: The addition of soy phosphatidylcholine to young rats increases the numbers of synapses and synaptic membranes in the rat spinal cord and increases the synaptic plasticity in the spinal cord of juvenile rats.