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植物名称:紫背万年青(Rhoeo discolor Hance),又名蚌花。材料类型:花茎。培养条件:(1) 诱导芽分化用MS+BA5mg/L(单位下同)+NAA0.5液体培养基(不加琼脂固化),培养材料以塑料泡沫作支持物。(2)诱导根分化用1/2MS+NAA0.5固体培养基。pH5.7±0.1,培养温度26±2℃,光照强度1000～1500Lx,每天光照12小时。生长与分化情况;1.诱导芽分化:切取带节花茎约1.0厘米长,经常规消毒后接种于(1)培养基中作静置培养,3天后侧芽开始生长,2～3周后,侧芽可长至1～2厘米高。此时
Plant Name: Purple evergreen (Rhoeo discolor Hance), also known as clam flowers. Material Type: Stems. Culture conditions: (1) induction of bud differentiation with MS + BA5mg / L (unit the same below) + NAA0.5 liquid medium (without agar solidification), the culture material to plastic foam as a support. (2) Induction of root differentiation with 1 / 2MS + NAA0.5 solid medium. pH5.7 ± 0.1, culture temperature 26 ± 2 ℃, light intensity 1000 ~ 1500Lx, light 12 hours a day. Growth and differentiation; 1. Induction of bud differentiation: Cut stems about 1.0 cm long, after conventional disinfection inoculated (1) culture medium for static culture, 3 days after the lateral buds began to grow, 2 to 3 weeks after the lateral buds Can grow to 1 to 2 cm high. at this time