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目的建立绝经期泌尿系感染动物模型。方法对小鼠进行去势和尿路感染处理,通过阴道细胞学涂片、阴道细胞角化指数、小鼠血清E2水平和去势小鼠膀胱细菌培养和膀胱病理分级对尿路感染结果进行评价。结果去势感染组和去势非感染组小鼠去势后5 d内阴道涂片全部出现并维持间情期表现,而假手术感染组和假手术非感染组小鼠术后5 d内阴道涂片则维持周期性变化;去势小鼠阴道脱落细胞角化指数表明,去势感染组和去势非感染组小鼠去势后比假手术感染组和假手术非感染组小鼠阴道脱落细胞角化指数明显减低;小鼠血清E2水平变化,去势感染组和去势非感染组小鼠去势后比假手术感染组和假手术非感染组明显减低(P<0.01);去势感染组和假手术感染组小鼠感染处理后膀胱尿培养及病理切片结果表明全部感染,而去势非感染组和假手术非感染组小鼠膀胱尿培养及病理切片结果则均为阴性。结论小鼠去势后进行膀胱感染,初步达到了绝经期泌尿系感染的动物模型程度。
Objective To establish an animal model of urinary tract infection in menopause. Methods Ovariectomy and urinary tract infection were performed in mice. The results of urinary tract infection were evaluated by vaginal cytology smears, vaginal keratinization index, serum E2 level in mice and bladder bacterial culture and bladder pathological grading in castrated mice . Results In vaginal smear and castration non-infected mice, all the vaginal smears appeared within 5 days after ovariectomy and maintained the inter-estrus performance. In the sham operation group and sham-operated non-infected group, the vagina Smear while maintaining the periodic changes; castrated mouse vaginal exfoliation cells keratosis index shows that the castration infected group and castrated non-infected mice than after sham operation group and sham-operated non-infected mice vaginal shedding (P <0.01). The number of keratinized cells was significantly decreased. The level of serum E2 in the mice was significantly lower than that in the sham-operated and non-infected groups (P <0.01) Infection group and sham operation group infected mice, bladder urinary culture and biopsy results showed that all infected, but castration non-infected group and sham-operated non-infected mice bladder urinary culture and pathological results were negative. Conclusions The bladder infection in mice after ovariectomy initially reached the level of animal model of menopausal urinary tract infection.