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以引自日本村田种苗公司的菜用羽衣甘蓝杂交种‘绿叶多汁’为试材,对小孢子胚诱导条件、胚状体转接时间、生芽培养基和生根培养基进行了优化筛选。在NLN-13+0.1mg·L-16-BA+0.1mg·L-1NAA和NLN-13+0.2mg·L-16-BA+0.2mg·L-1NAA两种培养基上获得了胚状体,其发生率分别为1.38胚.蕾-1和0.98胚.蕾-1;转胚时间以接种培养后25d为宜;适宜的胚状体生芽培养基为MS+2.0mg·L-16-BA+0.1mg·L-1NAA+3%蔗糖+0.7%琼脂,丛生芽诱导率为56.38%;生根培养基以1/2MS+0.1mg·L-1NAA+3%蔗糖+0.7%琼脂为宜,生根率100%。
In this study, the optimum conditions for the induction of microspore embryos, the transfer time of embryoid bodies, the germination medium and the rooting medium were screened from the kale hybrid jujube . Embryoid bodies were obtained on both NLN-13 + 0.1 mg · L-16-BA + 0.1 mg · L-1 NAA and NLN-13 + 0.2 mg · L-16-BA + 0.2 mg · L -1 NAA , The incidence rates were 1.38 embryos, -1 buds and 0.98 embryos, respectively. The time of embryo transfer was 25 days after inoculation. The suitable germination medium of embryoid bodies was MS + 2.0 mg · L-16- BA + 0.1 mg · L-1 NAA + 3% sucrose + 0.7% agar, the induction rate of cluster shoots was 56.38%; rooting medium with 1/2 MS + 0.1 mg · L -1 NAA + 3% sucrose + 0.7% The rooting rate of 100%.