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目的为充分利用有限的优质动物资源,缩短世代间隔,提高胚胎工程技术在生物领域中的应用效率。方法利用未性成熟小鼠模型和性成熟小鼠模型,分别进行PMSG/hCG超数排卵处理与体外受精;统计排卵率、受精率和囊胚发育率;观察小鼠卵巢的组织形态学变化,了解日龄对小鼠卵母细胞体外受精效果的影响,同时分析遗传因素在小鼠卵母细胞体外受精中的作用。结果未性成熟小鼠超数排卵的可用卵母细胞数量显著多于性成熟小鼠,且其卵巢上有腔卵泡的数量也明显多于性成熟小鼠;未性成熟小鼠卵母细胞的体外受精率显著低于性成熟小鼠,但囊胚发育率差异不显著。通过对性成熟小鼠的可用卵母细胞数进一步分析发现,高排卵组(平均可用卵母细胞数≥40枚)小鼠卵母细胞的体外受精率和囊胚发育率都显著高于低排卵组(平均可用卵母细胞数<40枚);且同窝小鼠的卵巢卵泡发育情况与排卵数基本一致。结论未性成熟小鼠卵巢对超数排卵反应的敏感性强,性成熟小鼠卵巢的超数排卵敏感性与遗传因素有关,且排卵性状的高低与卵母细胞的质量密切相关。
The purpose is to make full use of a limited number of high-quality animal resources, shorten the generation of intervals, improve the application of embryonic engineering technology in the field of biological efficiency. Methods The ovulation rate, fertilization rate and blastocyst rate of PMSG / hCG were detected by using the model of immature mice and sexual maturity mouse model respectively. The ovarian tissue morphological changes were observed, To understand the effects of age on in vitro fertilization of mouse oocytes, and to analyze the role of genetic factors in in vitro fertilization of mouse oocytes. Results The number of oocytes available for superovulation in immature mice was significantly more than that of sexually mature mice, and the number of antral follicles in ovaries was significantly more than that in sexually mature mice. The number of oocytes in immature mice In vitro fertilization rate was significantly lower than the sexual maturity mice, but the blastocyst development rate difference was not significant. Further analysis of available oocytes in sexually mature mice showed that the in vitro fertilization rate and blastula development rate of oocytes in the high ovulation group (mean number of available oocytes ≥ 40) were significantly higher than those in the low ovulation Group (the average number of available oocytes <40); and ovariectomized mouse follicular development and ovulation basically the same. Conclusion The ovary of immature mice is highly sensitive to superovulation, and the hypersensitivity of ovary in sexually mature mice is related to genetic factors. The ovulation trait is closely related to the quality of oocytes.