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应用双歧双歧杆菌作为量子点输送载体,为小动物在体生物肿瘤成像提供依据.采用电穿孔方法,将二棕榈酰磷脂酰胆碱(DPPC)包被的硫硒镉水溶性量子点转入细菌内,得到“量子点-细菌”复合探针,在“量子点-细菌”表面通过1-(3-二甲氨基丙基)-3-乙基碳二亚胺(EDC)活化法进一步偶联叶酸分子,制得“量子点-细菌-叶酸”复合纳米生物探针.将纳米生物探针经尾静脉注入Lewis肺癌小鼠体内,采用冰冻组织切片,考察探针在小鼠体内脏器及肿瘤的分布情况.结果表明,在肿瘤部位检测到较强的量子点光致发光信号,而在肺、肝、脾等脏器中只检测到微弱的量子点发光信号.“量子点-细菌-叶酸”复合纳米生物探针小动物在体肿瘤靶向成像是可行的.
Bifidobacterium bifidum was used as a quantum dot delivery carrier to provide a basis for the imaging of small animals in vivo.Using the electroporation method, dipalmitoylphosphatidylcholine (DPPC) coated sulfur, selenium and cadmium water-soluble quantum dots were transferred Into the bacteria to get “quantum dot - bacteria” composite probe, the “quantum dot - bacteria” surface by 1- (3- dimethylaminopropyl) -3-ethyl carbodiimide (EDC ) Activation method was further coupled to folic acid molecules to prepare “quantum dot - bacteria - folic acid ” composite nano-bio-probes.Micro-biological probes were injected into the tail vein of Lewis lung cancer mice, using frozen tissue sections, In vivo distribution of organs and tumors in mice.The results showed that strong quantum dot photoluminescence signal was detected in the tumor site and only weak quantum dot luminescence signal was detected in organs such as lung, liver and spleen . “Quantum Dot - Bacterial - Folic Acid” Composite Nanobiology Probiotics Targeting Imaging in Tumor Targeting is Possible.