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目的对新疆维药阿里红中的多糖进行提取,并研究阿里红粗多糖体外免疫活性。方法建立热水提取、乙醇沉淀、Sevag法除蛋白的方法提取阿里红多糖(FoP),红外及紫外吸收光谱检测其性质,采用苯酚-硫酸法测定多糖含量;采用MTT法检测不同浓度FoP在体外对小鼠脾细胞及腹腔巨嗜细胞增殖率的影响。结果 FoP产率为0.85%,多糖含量为68.81%;IR谱中,3423,2929,1651,1456,1130,1091,617cm-1处表现为典型的多糖吸收峰;UV谱中,260,280nm处均无吸收峰;体外活性试验中,FoP对小鼠脾细胞、腹腔巨嗜细胞的生长具有明显的促进作用。结论 FoP具有一定的免疫调节活性。
Objective To extract the polysaccharides of Xinjiang Uygur medicine, and to study the in vitro immunological activity of the polysaccharides of Alisma orientalis. Methods The extraction method of hot water, ethanol precipitation and Sevag method were used to extract the polysaccharides from Alhumoderma lucidum polysaccharides (FoP). The properties of the polysaccharides were determined by infrared and ultraviolet absorption spectroscopy. The content of polysaccharides was determined by phenol-sulfuric acid method. On mouse spleen cells and peritoneal macrophage proliferation rate. The results showed that the yield of FoP was 0.85% and the content of polysaccharide was 68.81%. In IR spectrum, 3423, 2929, 1651, 1456, 1130, 1091 and 617 cm- No absorption peak; In vitro activity test, FoP on mouse spleen cells, peritoneal macrophages growth has a significant role in promoting. Conclusion FoP has some immunomodulatory activity.