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目的:建立同时测定紫草中咖啡酸四聚体及其异构体含量的高效液相色谱法。方法:色谱柱为YMC-Pack ODS-AC18(250mm×4.6mm,5μm),流动相:甲醇-0.5%磷酸水(40∶60);流速:1.0mL·min-1;检测波长:252nm,柱温:室温;进样量:10μL。结果:咖啡酸四聚体在0.065～1.82μg,咖啡酸四聚体异构体在0.052～1.65μg范围内与峰面积值呈良好的线性关系;咖啡酸四聚体和咖啡酸四聚体异构体的平均回收率分别为98.72%(RSD为3.27%,n=9),101.8%(RSD为2.46%,n=9)。结论:本方法简便可靠,结果稳定,重复性好,可作为紫草质量控制方法之一。
Objective: To establish a method for simultaneous determination of caffeic acid tetramers and their isomers in Lithospermum erythrorhizon by HPLC. Methods: The chromatographic column was YMC-Pack ODS-AC18(250mm×4.6mm, 5μm), mobile phase: methanol-0.5% phosphoric acid water (40∶60); flow rate: 1.0mL·min-1; detection wavelength: 252nm, column Temperature: room temperature; injection volume: 10 μL. Results: The caffeic acid tetramer was in the range of 0.065-1.82 μg, and the caffeic acid tetramer isomer in the range of 0.052-1.65 μg showed a good linear relationship with the peak area value; the caffeic acid tetramer and the caffeic acid tetramer were different. The average recoveries of the structures were 98.72% (RSD 3.27%, n=9) and 101.8% (RSD 2.46%, n=9). Conclusion: This method is simple, reliable, stable, and reproducible. It can be used as one of the quality control methods for purple grass.