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目的 研究肿瘤坏死因子 -α(TNF -α)、白细胞介素 - 1(IL - 1)对体外培养的人子宫内膜间质细胞生长的调节和对间质细胞泌乳素 (PRL)产生的影响 ,探讨TNF -α、IL - 1对女性生殖生理的调节作用。方法 MTT比色法和放射免疫技术测定体外培养的人子宫内膜间质细胞的增殖和PRL的产生。结果 TNF -α、IL - 1均能抑制子宫内膜间质细胞的增殖及PRL的产生 ,并随浓度增加抑制作用增强。结论 TNF -α、IL - 1调节子宫内膜的功能变化 ,参与妊娠维持及子宫的病理变化。
Objective To investigate the effects of tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1) on the growth of human endometrial stromal cells cultured in vitro and on the production of prolactin (PRL) To investigate the regulatory effect of TNF-α and IL-1 on female reproductive physiology. Methods MTT assay and radioimmunoassay were used to detect the proliferation of human endometrial stromal cells cultured in vitro and PRL production. Results Both TNF - α and IL - 1 could inhibit the proliferation of endometrial stromal cells and the production of PRL, and their inhibitory effects were enhanced with the increase of concentration. Conclusion TNF - α and IL - 1 regulate the functional changes of endometrium and participate in the maintenance of pregnancy and pathological changes of the uterus.