论文部分内容阅读
γ-生育酚在北美人膳食生育酚中占的比例很大,其生物活性可能是α形式的2倍。但许多实验室的结果表明,人血浆和血细胞中的α-生育酚高于γ-生育酚,二者相比,比例范围为3.4~6.5,提示2种生育酚的代谢速率是不同的,γ-生育酚的贮留低于α生育酚。一般认为,在体内γ-生育酚的活性低于α-生育酚,仅为β-生育酚的10~20%,但在体外,γ-生育酚的抗氧化活性要比α-生育酚强,因此生育酚的生物活性与它们的抗氧化活性并不一致。本文对γ-生育酚被人体内皮细胞摄入或消除的情况作了研究,并就γ-和α-生育酚的摄入作了比较。将培养至亚融合状态的人脐静脉血管内皮细胞单层作为被试物。γ-生育酚先溶解至DMSO 中,再加到灭活的胎牛血清中,然后与培养基的其它成分混合成为处理组培养基,对照组的培养基中仅添加的量不足培养基总量1%的 DMSO。根据 Bigh 和 Dyer 的方法提取
Gamma-tocopherol is a large proportion of dietary tocopherols in North Americans, and its bioactivity may be twice that of the alpha form. However, the results of many laboratories show that α-tocopherol in human plasma and blood cells is higher than γ-tocopherol, with a ratio ranging from 3.4 to 6.5, suggesting that the metabolic rates of the two tocopherols are different and γ - Tocopherol storage is lower than alpha tocopherol. It is generally believed that the activity of γ-tocopherol in the body is lower than that of α-tocopherol, only 10 to 20% of that of β-tocopherol. However, in vitro, γ-tocopherol has stronger antioxidant activity than α-tocopherol, Therefore, the biological activity of tocopherols and their antioxidant activity are not the same. In this paper, γ-tocopherol was ingested or eliminated by human endothelial cells, and the intake of γ- and α-tocopherol was compared. Human umbilical vein endothelial cell monolayers cultured to sub-fusion state were used as test subjects. γ-tocopherol was first dissolved in DMSO and added to inactivated fetal bovine serum, and then mixed with the other components of the culture medium to become the treatment group medium. The amount added to the culture medium in the control group was less than the total amount of the medium 1% DMSO. Extract according to Bigh and Dyer’s method