论文部分内容阅读
目的研究蛋白激酶C(proteinKinaseC,PKC)和蛋白激酶A(proteinkinaseA,PKA)在甲状腺癌发生过程中的作用。方法利用酶的放射分析方法测定手术切除经病理证实的18例甲状腺癌患者和17例甲状腺癌患者手术切除的肿瘤组织中PKC和PKA活性水平。结果甲状腺癌组织中胞膜和胞浆PKC活性分别为7.280±2.380和7.397±4.065,PKA活性水平为1.716±0.923。甲状腺癌组织中胞膜和胞浆PKC活性分别为0.515±0.676和0.347±0.332,PKA活性水平为0.646±0.324。胞浆部分PKC与PKA活性比值:甲状腺癌组织为4.3;甲状腺癌组织为0.53。结论PKC与PKA均参入甲状腺癌细胞的增殖过程。
Objective To study the role of protein kinase C (PKC) and protein kinase A (PKA) in the pathogenesis of thyroid carcinoma. Methods The levels of PKC and PKA in surgical resected tumor tissues of 18 cases of thyroid carcinoma and 17 cases of thyroid carcinoma confirmed by pathology were determined by radioimmunoassay. Results The cytoplasmic and cytoplasmic PKC activities in thyroid cancer tissues were 7.280 ± 2.380 and 7.397 ± 4.065, respectively. The PKA activity level was 1.716 ± 0.923. The cytoplasmic and cytoplasmic PKC activities in thyroid cancer tissues were 0.515 ± 0.676 and 0.347 ± 0.332, respectively, and the PKA activity level was 0.646 ± 0.324. Cytoplasmic PKC and PKA activity ratio: thyroid cancer was 4.3; thyroid cancer was 0.53. Conclusions Both PKC and PKA participate in the proliferation of thyroid carcinoma cells.