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本文着重进行了胃癌的荧光分析。从胃癌组织匀浆,胃液,胃癌患者的血清中检测与其相关的标志物。通过对标志物荧光的检测,进行胃癌临床诊断研究。一、实验部分(一)组织匀浆的制备分取胃癌组织匀浆,胃癌非癌粘膜组织匀浆,胃溃疡胃粘膜组织匀浆和十二指肠组织匀浆四组。将手术中切除的新鲜癌组织切取2克;同时切取距癌组织边缘5厘米以上非癌粘膜2克,取胃溃疡及十二指肠溃疡的病人正常粘膜各2克,切碎,分别加入生理盐水20毫升在0℃用组织匀浆器将该组织制成匀浆,离心,取上层清液测试。
This article focuses on the fluorescence analysis of gastric cancer. From the serum of gastric cancer homogenates, gastric juices, and gastric cancer patients, their associated markers were detected. Through the detection of fluorescent markers, clinical diagnosis of gastric cancer was studied. First, the experimental part (a) the preparation of tissue homogenates were divided into gastric cancer tissue homogenates, gastric cancer non-cancer mucosal tissue homogenates, gastric ulcer gastric mucosal tissue homogenate and duodenal tissue homogenate four groups. Two grams of fresh cancerous tissue removed during surgery were removed. At the same time, 2 grams of non-cancerous mucosa above 5 cm from the edge of the cancerous tissue were cut. Two grams of normal mucosa were taken from patients with gastric ulcer and duodenal ulcer. 20 ml of saline was homogenized with a tissue homogenizer at 0°C, centrifuged, and the supernatant was tested.