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BACKGROUND:The present study investigated the effects of low-molecular-weight components of bovine colostrum (LMCC),which were administered per os,on the differentiation,growth,and survival of cells obtained from the bone marrow of rats in primary culture.METHODS:Bone marrow cells (BMCs) were obtained from both the rat femurs and were cultured in medium 199 supplemented with antibiotics (8% streptomycin and 8% gentamycin) and 20% inactivated fetal calf serum.In addition,the number of BMCs was counted and their morphotypes were determined.RESULTS:Animals were treated with copper (Cu) sulfate to induce liver fibrosis.Subsequent treatment with LMCC eliminated growth inhibition and normalized the bodyweight and temperature of animals with Cu-induced liver fibrosis.The number of lymphocytes in the bone marrow of animals with Cu-induced liver fibrosis was significantly higher than that in the bone marrow of control animals.The number ofneutrophils in untreated animals with liver fibrosis and LMCC-treated animals with liver fibrosis was lower than that in control animals.Neutrophils obtained from untreated animals with liver fibrosis and LMCC-treated animals with liver fibrosis underwent two-times faster degradation in vitro than neutrophils obtained from control animals.CONCLUSIONS:Our results indicate that LMCC affects the distribution of different morphological types of BMCs but does not prevent their degradation in vitro,which was two-times faster than that of BMCs obtained from control animals.