论文部分内容阅读
目的通过颈动脉注射血吸虫虫卵建立家兔脑肉芽肿模型。方法实验组家兔10只,行全脑放疗(20GY,单次照光)破坏血脑屏障;照光后7d,通过颈动脉注射血吸虫虫卵悬浮液1ml建模。阴性对照组10只,全脑放疗后7d经颈动脉注射生理盐水1ml。空白对照组10只,不进行任何处理。术后使用抗生素预防感染,观察3组动物临床表现。30d后行MRI检查。取脑组织做病理切片,观察脑组织大体标本及病理改变。结果实验组2只、阴性对照组1只家兔死于放疗后并发症。实验组8只家兔术后均出现轻度食欲不振,体重下降,1周后症状逐渐消失;8只出现呆滞、嗜睡等精神症状。阴性对照组家兔术后均食欲下降,1周后症状消失,无异常精神症状。空白对照组未见异常。MRI检查实验组增强T1WI见脑膜线状强化4例,脑组织内见结节状强化2例。对照组未见明显异常。脑组织大体标本及病理切片检查实验组8只家兔均出现血管炎,7只出现脑水肿,6只病理切片见肉芽肿结节,5只出现脑膜炎。阴性对照组及空白对照组未见脑水肿及肉芽肿结节(阴性对照组1只出现血管周围炎)。结论经颈动脉注射血吸虫虫卵建立的家兔脑肉芽肿模型出现相应的组织病理变化及临床表现,符合实验要求。
Objective To establish rabbit brain granuloma model by injecting Schistosoma japonicum eggs through carotid artery. Methods Totally 10 rabbits in the experimental group were subjected to whole brain irradiation (20GY, single light) to destroy the blood-brain barrier. Seven days after irradiation, 1ml of Schistosoma japonicum egg suspension was injected through the carotid artery. Negative control group of 10, whole brain 7d after radiotherapy through the carotid artery injection of normal saline 1ml. Blank control group of 10, without any treatment. Antibiotics were used to prevent infection after operation, and the clinical manifestations of the three groups were observed. After 30d MRI examination. Take brain tissue sections, observe the gross brain tissue and pathological changes. Results Two experimental groups and one negative control group died of complications after radiotherapy. Eight rabbits in the experimental group showed slight loss of appetite and weight loss after operation, and gradually disappeared after one week. Eight showed symptoms of sluggishness and drowsiness. Negative control group rabbits appetite decreased after 1 week, the symptoms disappeared, no abnormal mental symptoms. No abnormal control group. MRI examination of the experimental group enhanced T1WI see meningeal line enhancement in 4 cases, brain tissue see nodular enhancement in 2 cases. The control group no obvious abnormalities. Gross brain tissue samples and pathological examination of the experimental group of 8 rabbits were vasculitis, cerebral edema occurred in 7, 6 pathological section of granuloma nodules, 5 meningitis. Negative control group and blank control group, no brain edema and granuloma nodules (negative control group, only one perivascular inflammation). Conclusions The brain tissue granuloma model in rabbits injected with schistosomiasis through carotid artery showed corresponding histopathological changes and clinical manifestations, which met the experimental requirements.