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目的 研究小鼠腹腔注射贝母乙素的药代动力学。方法 反相液相色谱法定量分析。血浆样品经乙醚提取后 ,柱前用2 ,4 二硝基苯肼衍生 ,分析色谱柱 :HypersilC18,流动相 :乙腈 乙酸铵 (0 .1mol·L-1,pH =5 ) (39∶6 1) ,UV375nm检测。结果 在0 .114～ 1.2 6 μg·ml-1区间 ,标准曲线线性方程为Y =0 .12 6X - 0 .0 0 13,相关系数r=0 .992 3,平均日间、日内精密度RSD分别为5 .85 %和 5 .30 %。平均相对回收率为 96 .5 %。结论 小鼠注射贝母乙素 (1.5mg·kg-1)的血药浓度 时间曲线符合一室开放模型 ,t1/ 2 为 0 .84h ,cmax为 0 .87μg·ml-1,tmax为 0 .15h。
Objective To study the pharmacokinetics of intraperitoneal injection of pemetrexin in mice. Methods Quantitative analysis by reversed-phase liquid chromatography. The plasma sample was extracted with ether and the column was derivatized with 2,4 dinitrophenylhydrazine. The analytical column was Hypersil C18 and the mobile phase was acetonitrile ammonium acetate (0.1 mol·L-1, pH = 5) (39:6 1). ), UV375nm detection. Results In the range of 0.114-1.2 6 μg·ml-1, the linear equation of the standard curve was Y =0.12 6X-0. 0 0 13. The correlation coefficient was r=0.992 3, the average daytime and intraday precision RSD. They were 5.85% and 5.30%, respectively. The average relative recovery was 96.5%. Conclusions The time profile of blood concentration in mice injected with ferritin (1.5 mg·kg-1) accords with the one-compartment open model, t 1 /2 is 0.84 h, cmax is 0. 87 μg·ml-1, and tmax is 0. 15h.