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目的研究微小RNA-138(microRNA-138,miR-138)对肝细胞癌(肝癌)细胞繁殖、侵袭转移的影响。方法在2015年3—12月实验将HepG2细胞分别转染miR-138类似物(miR-138 mimic)及miR-阴性对照(negative control,NC),实时荧光定量PCR技术(real time quantitative PCR,qRT-PCR)检测miR-138表达改变,MTT实验检测HepG2细胞繁殖情况,Transwell实验检测HepG2细胞侵袭转移能力。组间差异采用t检验,P<0.05为差异有统计学意义。结果 qRT-PCR检测结果证实转染miR-138 mimic组HepG2细胞miR-138表达为转染miR-NC组(25.53±5.12)倍,对比差异有统计学意义(P<0.05)。经MTT、Transwell实验发现miR-138 mimic组HepG2细胞繁殖及侵袭转移能力明显下降。结论 miR-138过表达能有效抑制HepG2细胞繁殖及侵袭转移。
Objective To investigate the effect of microRNA-138 (miR-138) on the proliferation, invasion and metastasis of hepatocellular carcinoma (HCC) cells. METHODS: HepG2 cells were transfected with miR-138 mimic and miR-negative control (NC), real-time quantitative PCR (qRT) -PCR) was used to detect the expression of miR-138. The proliferation of HepG2 cells was detected by MTT assay and the invasion and metastasis of HepG2 cells by Transwell assay. Differences between groups using t test, P <0.05 for the difference was statistically significant. Results The results of qRT-PCR confirmed that the expression of miR-138 in HepG2 cells transfected with miR-138 mimic group was 25.53 ± 5.12 times of that of miR-138 group transfected with miR-138, the difference was statistically significant (P <0.05). The MTT and Transwell experiments showed that the proliferation and invasion and metastasis of HepG2 cells in miR-138 mimic group were significantly decreased. Conclusion miR-138 overexpression can effectively inhibit HepG2 cell proliferation and invasion and metastasis.