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目的:以实验室前期研究得到的微乳处方为基础,采用乳糜微粒阻断法研究微乳处方中不同链长度油相对葛根素微乳经淋巴转运的影响。方法:采用乳糜微粒阻滞技术阻断药物淋巴转运,高效液相色谱法测定大鼠血浆中葛根素浓度,比较阻断组与未阻断组生物利用度来计算淋巴转运的比例。结果:长链油微乳的淋巴转运比例为49.7%,中链油微乳、及短链油微乳为29.8%、22.5%。长链油微乳的淋巴转运比例高于中链油微乳及短链油微乳,且其生物利用度也高于中链油微乳及短链油微乳,差异具有统计学意义。结论:长链油微乳更能促进葛根素经肠道吸收,增加其在血浆中的浓度,从而提高生物利用度。
OBJECTIVE: To study the effects of different chain lengths of oil on puerarin microemulsion via the chylomicron blocker method, based on the microemulsion prescription obtained in previous laboratory studies. METHODS: Chylomicron blockade was used to block the lymphatic transport of drugs. The plasma puerarin concentration was determined by high performance liquid chromatography (HPLC), and the ratio of lymphatic transit was calculated by comparing the bioavailability between blocking and non-blocking groups. Results: The long-chain oil microemulsion lymphatic transport ratio was 49.7%, medium-chain microemulsion, and short chain microemulsion 29.8%, 22.5%. The lymphatic transport of long-chain oil microemulsion was higher than that of medium-chain microemulsion and short-chain microemulsion, and its bioavailability was also higher than that of medium-chain microemulsion and short-chain microemulsion. The difference was statistically significant. Conclusion: The long-chain oil microemulsion can promote the absorption of puerarin by intestinal tract and increase the concentration of puerarin in plasma, so as to improve the bioavailability.