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目的:探讨绞股蓝总皂苷(Gypenosides,Gyp)对小鼠白血病L1210细胞生长抑制的作用机制。方法:采用MTT法检测Gyp对体外培养的小鼠L1210细胞的增殖抑制作用,流式细胞仪检测Gyp对L1210细胞线粒体膜电位的影响;DAPI染色检测Gyp对L1210白血病细胞核的变化;单细胞凝胶电泳法检测Gyp对白血病L1210细胞DNA损伤的影响。结果:浓度为100～500μg/mL的Gyp对L1210细胞增殖生长有抑制作用,350μg/mL的Gyp作用于L1210细胞0、12、24 h,线粒体膜电位显著下降;Gyp(350μg/mL)作用4 h时,细胞的活性氧产量最高。Gyp(350μg/mL)作用4、12、24 h后,单细胞凝胶电泳观测到DNA损伤;DAPI染色发现细胞核发生变化,并有时间依赖性。结论:Gyp能抑制L1210细胞增殖,这种抑制作用与活性氧的产生、线粒体膜电位下降和DNA损伤有关。
Objective: To investigate the mechanism of Gypenosides (Gyp) on the growth inhibition of mouse leukemia L1210 cells. Methods: MTT assay was used to detect the effect of Gyp on the proliferation of L1210 cells in vitro. Flow cytometry was used to detect the effect of Gyp on the mitochondrial membrane potential of L1210 cells. DAPI staining was used to detect the changes of nuclei in L1210 leukemia cells. Effect of Gyp on DNA damage of leukemia L1210 cells by electrophoresis. Results: Gyp at concentration of 100-500μg / mL inhibited the proliferation and proliferation of L1210 cells. 350μg / mL of Gyp inhibited the mitochondrial membrane potential at 0, 12 and 24 hours in L1210 cells. Gyp (350μg / mL) h cells, the highest production of reactive oxygen species. Gyp (350μg / mL) for 4,12,24 h after single cell gel electrophoresis DNA damage was observed; DAPI staining found nuclear changes in a time-dependent manner. Conclusion: Gyp can inhibit the proliferation of L1210 cells. This inhibitory effect is related to the production of reactive oxygen species, mitochondrial membrane potential and DNA damage.