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目的利用携带SSTR2基因的重组腺病毒载体Adeno-X-SSTR2转染裸鼠胰腺癌皮下模型,使用SSTR2特异激动剂NC-8-12,观察其在体内实验中是否能取得抑制胰腺癌细胞增殖的效果。方法构建重组携带SSTR2基因的肿瘤增殖缺陷型腺病毒载体Adeno-X-SSTIL2,建立裸鼠胰腺癌皮下模型,分为转染和未转染SSTR2基因的两组,皮下分别给予不同剂量NC-8-12,观察肿瘤体积和重量的变化。结果在裸鼠皮下模型实验中。NC-8-12对未转染SSTR2基因的胰腺癌细胞无抑制作用(P＞0．05)；在转染了SSTR2基因的胰腺癌细胞中。NC-8-12在每日200μg/kg体重剂量下可以明显抑制肿瘤细胞增殖(P＜0．05)。结论(1)携带SSTR2基因的肿瘤增殖缺陷型腺病毒载体Adeno-X-SSTR2可以高效转染胰腺癌细胞株。(2)一定剂量的NC-8-12(每日200μg/kg体重)对裸鼠体内转染了SSTR2基因的胰腺癌细胞的生长有明显抑制作用。
Objective To transfect the subcutaneous model of pancreatic cancer in nude mice with the recombinant adenoviral vector Adeno-X-SSTR2 carrying the SSTR2 gene and to use SSTR2-specific agonist NC-8-12 to observe whether it can inhibit the proliferation of pancreatic cancer cells in vivo. effect. Methods The recombinant adenovirus vector Adeno-X-SSTIL2 carrying the SSTR2 gene was constructed and reconstructed. The subcutaneous model of pancreatic cancer in nude mice was established and divided into two groups: transfected and untransfected SSTR2 gene. Different doses of NC-8 were given subcutaneously. -12, observe changes in tumor volume and weight. The results were in a subcutaneous model experiment in nude mice. NC-8-12 had no inhibitory effect on pancreatic cancer cells that had not been transfected with the SSTR2 gene (P>0.05); in pancreatic cancer cells transfected with the SSTR2 gene. NC-8-12 significantly inhibited tumor cell proliferation at a daily dose of 200 μg/kg body weight (P<0.05). Conclusions (1) The adeno-X-SSTR2 adenovirus vector carrying the SSTR2 gene can efficiently transfect pancreatic cancer cell lines. (2) A certain dose of NC-8-12 (200μg/kg body weight per day) significantly inhibited the growth of pancreatic cancer cells transfected with SSTR2 gene in nude mice.