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生长抑制试验是评价疟疾保护性抗体的一种方法。抗体对同株的恶性疟原虫的抑制作用比异株的强。最近的研究表明,疟疾流行区居民的血清在体外与~3H-异白氨酸混合能抑制疟原虫的生长,这种抑制作用大多是由抗体而不是由抗疟药物所引起。作者采用3种恶性疟原虫虫株,即SO、SN和G-112株供实验用。在非传染季节里,对居住在泰国北碧省的疟疾流行区(当时血片阳性率为2%)的67人采取静脉血,另60个对照血清取自无疟疾既往史的曼谷献血者,所得血清于-70℃中保存直至使用。生长抑制试验是根据1969年Cohen等叙述的
Growth inhibition test is a method of evaluating protective antibodies against malaria. Antibodies against the same strain of Plasmodium falciparum than the stronger than the inhibition. Recent studies have shown that the serum of residents in malaria endemic areas inhibits the growth of Plasmodium by mixing with 3H-isoleucine in vitro, mostly by antibodies rather than antimalarial drugs. The authors used three P. falciparum strains, SO, SN and G-112, for experimental use. In the non-contagious season, venous blood was collected from 67 people living in malaria-endemic areas of Kanchanaburi Province of Thailand (2% positive blood-flakes at that time), and the other 60 control sera were taken from Bangkok blood donors without previous malaria history. The resulting serum was stored at -70 ° C until use. The growth inhibition test is based on what Cohen et al. (1969) described