论文部分内容阅读
目的:探讨银杏叶提取物(extract of Gingko biloba leaves,EGB)对衰老小鼠学习、记忆障碍的改善作用及其作用机制。方法:将33只雄性小鼠分为3组,每组11只。即模型组:按300mg/kgBW剂量腹腔注射D-半乳糖,1次/d;治疗组:在按上述剂量注射D-半乳糖的同时,按15mg/kgBW剂量腹腔注射EGB,1次/d;对照组:按上述同样剂量注射生理盐水溶液,1次/d。所有实验处理进行6周后停止给药,用Y式迷宫、跳台法检测小鼠的学习记忆能力,同时测定肝、脑组织的单胺氧化酶(monoamine oxidase,MAO)活性。结果治疗组的学习、记忆成绩均显著高于模型组,差异都具有统计学意义(P<0.05或P<0.01)。结论:EGB对于衰老小鼠的学习、记忆障碍有明显的改善作用,在其作用机制中,MAO发挥了重要作用。
Objective: To investigate the effect of extract of Gingko biloba leaves (EGB) on learning and memory impairment in aging mice and its mechanism of action. Methods: Thirty-three male mice were divided into 3 groups, 11 in each group. The model group: according to the 300mg/kgBW dose of intraperitoneal injection of D-galactose, once / d; treatment group: at the same time as the above dose of D-galactose injection, according to 15mg/kgBW dose of intraperitoneal injection of EGB, once / d; Control group: A physiological saline solution was injected at the same dose as described above, once a day. Six weeks after all experimental treatments were stopped, the mice’s learning and memory abilities were measured using a Y-maze and a step-by-step method. Monoamine oxidase (MAO) activity in liver and brain tissues was also measured. Results The learning and memory scores of the treatment group were significantly higher than those of the model group, and the differences were statistically significant (P<0.05 or P<0.01). Conclusion: EGB has a significant effect on improving learning and memory impairment in aging mice. In its mechanism of action, MAO plays an important role.