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小儿急性中耳炎,有13～50%患儿未能在其中耳渗出液中找到细菌。作者们采用新开展的酶联免疫吸附法(ELISA)研究病毒与中耳炎发病的关系,对象为约翰·霍浦金斯医院儿科门诊确诊的53例未经治疗的中耳炎患者,年龄2月～12岁。每例除详细记录病史外,还作诊断性鼓室穿刺。将中耳抽吸液、鼻咽冲洗液、外耳拭子及大便标本均作了多种病原学检查。有45例在起病时与病后10～60天分别收集血清,以测定抗呼吸道合胞病毒(RSV)抗体,凡抗体滴度升高4倍以上者,为RSV感染之血清学诊断依据。结果:在53例中,明确病原体者34例(64%),依次为肺炎链球菌17例(32%),
Pediatric acute otitis media, 13 to 50% of children failed to find bacteria in the middle ear exudate. The authors used a newly developed enzyme-linked immunosorbent assay (ELISA) to study the relationship between the virus and the incidence of otitis media for 53 cases of untreated otitis media diagnosed at pediatric outpatient Johns Hopkins Hospital, aged from 2 months to 12 years . In addition to detailed records of each case history, but also for diagnostic tympanic puncture. A variety of etiological tests were performed on the middle ear aspirate, nasopharyngeal wash, outer ear swabs and stool specimens. Serum samples were collected from 45 patients at the time of onset and 10 to 60 days after the onset of illness to determine anti-RSV antibodies. Serological diagnosis of RSV infection was based on a 4-fold increase in antibody titer. Results: In 53 cases, 34 cases (64%) were confirmed as pathogens, followed by 17 cases (32%) of Streptococcus pneumoniae,