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目的信号转导和转录激活蛋白5(signal transduction and transcriptional activation protein,STAT 5),包括STAT 5a和STAT 5b,被认为在肿瘤的多种生物学特性中具有重要作用。本研究旨在检测胃癌组织STAT 5b的表达,分析STAT 5b在胃癌细胞对吉非替尼化疗敏感性中的作用及可能机制。方法选取2013-03-01-2013-12-31在山东大学附属千佛山医院住院的69例原发性胃癌患者作为研究对象。免疫组化法检测69例胃癌患者癌和癌旁组织中STAT5b的表达,体外培养胃癌MGC-803和MKN-45细胞,分别暴露在吉非替尼和(或)STAT 5bsiRNA中。结果胃癌组织STAT 5b阳性表达率为71.0%(49/69),胃癌旁组织中阳性表达率为39.1%(27/69),差异有统计学意义,P=0.001。4mmol/L吉非替尼作用24h后MGC-803和MKN-45的存活率分别为(54.01±6.72)%和(56.13±5.02)%,明显降低且呈现时间和浓度依赖性;细胞凋亡率分别为(5.1±1.1)%和(5.9±1.3)%,与对照组(2.1±1.2)%和(1.6±1.1)%相比显著升高,P<0.05;STAT 5b的蛋白表达水平显著下调。STAT 5bsiRNA协同吉非替尼作用MGC-803和MKN-45后的细胞凋亡率分别为(23.4±3.2)%和(19.7±3.16)%,显著低于STAT 5bsiRNA和吉非替尼单独作用组。结论与癌旁组织比较,STAT 5b在胃癌组织中的表达显著升高,干扰STAT 5b的表达能够增强胃癌细胞对吉非替尼的化疗敏感性,其机制与促进细胞凋亡有关。
Objective Signal transduction and transcriptional activation protein (STAT 5), including STAT 5a and STAT 5b, are thought to play an important role in the diverse biological properties of tumors. This study aimed to detect the expression of STAT 5b in gastric cancer tissues and to analyze the role of STAT 5b in the sensitivity of gastric cancer cells to gefitinib chemotherapy. Methods 2013-03-01-2013-12-31 Selected 69 patients with primary gastric cancer hospitalized in Qianfoshan Hospital Affiliated to Shandong University as the research object. The expression of STAT5b was detected by immunohistochemistry in 69 cases of gastric cancer and paracancerous tissues. The gastric cancer MGC-803 and MKN-45 cells were cultured in vitro and exposed to gefitinib and / or STAT5bsiRNA respectively. Results The positive rate of STAT 5b in gastric cancer tissue was 71.0% (49/69), while the positive rate in gastric cancer tissues was 39.1% (27/69), the difference was statistically significant (P = 0.001.4 mmol / L) The survival rates of MGC-803 and MKN-45 after 24 h treatment were (54.01 ± 6.72)% and (56.13 ± 5.02)%, respectively, which were significantly decreased and showed time- and concentration- % And (5.9 ± 1.3)% respectively, which was significantly higher than those in the control group (2.1 ± 1.2)% and (1.6 ± 1.1)%, P <0.05; STAT 5b protein expression was significantly downregulated. The apoptotic rates of STAT 5bsiRNA and Gefitinib after MGC-803 and MKN-45 treatment were (23.4 ± 3.2)% and (19.7 ± 3.16)%, respectively, which were significantly lower than those of STAT 5bsiRNA and gefitinib alone . Conclusions The expression of STAT 5b in gastric cancer tissues is significantly higher than that in adjacent non-cancerous tissues. Interfering STAT 5b expression enhances the chemosensitivity of gastric cancer cells to gefitinib, which is related to the promotion of apoptosis.