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实验动物采用体重为295克～430克、耳廓反射阳性的白化豚鼠。经左侧枕后进路于硬膜外,将乙状窦从颞骨内表面分离并牵向内侧,恰好在乙状窦前方暴露内淋巴囊(ES)。用注满154mmol/L NaCl的玻璃显微电极插入ES中部来测ESP,参考电极置于颈部肌肉。全部实验都在电屏蔽室内进行。ESP值是指在ES周围的组织液与在ES内部之间所记录到的电位变化量。乙酰唑胺溶液于2ml盐水中用注入泵在1分钟内经插管注入颈静脉。结果:①乙酰唑胺在0.08mg/kg剂量时,ESP振幅变化不明显,但在1.0mg/kg及10mg/kg时,则有明显下降,下降开始于注药后,在20分钟时接近趋于稳定。②在小于10mg/kg时,ESP振幅的下降有明显
Experimental animals using body weight of 295 grams to 430 grams, auricle reflex positive albino guinea pigs. After the left occipital posterior approach in the epidural, the sigmoid sinus separated from the temporal bone surface and toward the inside, just in front of sigmoid sinus exposed endolymphatic sac (ES). The ESP was placed in the middle of the ES with a glass microelectrode filled with 154 mmol / L NaCl and the reference electrode was placed on the neck muscles. All experiments were conducted in electrically shielded rooms. The ESP value is the amount of change in potential recorded between the tissue fluid surrounding the ES and the inside of the ES. Acetazolamide solution was injected via cannula into the jugular vein in 2 ml of saline using an infusion pump over a period of 1 minute. RESULTS: Acetazolamide at 0.08 mg / kg had no significant changes in the amplitude of ESP, but at 1.0 mg / kg and 10 mg / kg, there was a significant decrease. The decline started at 20 minutes after infusion Stable. ② less than 10mg / kg, ESP amplitude decreased significantly